Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer-Liposome Aggregates

Biplab Roy, Pritam Guha, Prasant Nahak, Gourab Karmakar, S. Maiti, A.K. Mandal, Борис Анатольевич Носков, Алексей Геннадьевич Быков, Александр Владимирович Акентьев, K. Tsuchiya, K* Torigoe, Amiya Kumar Panda

Research output

1 Citation (Scopus)

Abstract

Interaction between negatively charged liposomes and cationic polyamidoamine dendrimers of different generations was investigated through size, zeta potential, turbidity, electron microscopy, atomic force microscopy, fluorescence spectroscopy, and calorimetric studies. Liposomes with the binary combination of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) + dihexadecyl phosphate, DPPC + 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol, DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphate, and DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol were stable up to 60 days. The electrostatic nature of dendrimer-lipid bilayer interaction was evidenced through charge neutralization and subsequent reversal upon added dendrimer to liposome. Dendrimer-liposome interaction depended on its generation (5 > 4 > 3) in addition to the charge, head groups, and hydrocarbon chain length of lipids. Fluorescence anisotropy and differential scanning calorimetry studies suggest the fluidization of the bilayer, although the surface rigidity was enhanced by the added dendrimers. Thermodynamic parameters of the interaction processes were evaluated by isothermal titration and differential scanning calorimetric studies. The binding processes were exothermic in nature. The enthalpy of transition of the chain melting of lipids decreased systematically with increasing dendrimer concentration and generation. Dendrimer-liposome aggregates were nontoxic to healthy human blood cell, suggesting the potential of such aggregates as drug delivery systems.

Original languageEnglish
Pages (from-to)12235-12245
Number of pages11
JournalACS Omega
Volume3
Issue number9
DOIs
Publication statusPublished - 30 Sep 2018

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Dendrimers
Liposomes
Phosphatidylcholines
Chemical analysis
Lipids
Phosphates
Lipid bilayers
Fluidization
Fluorescence spectroscopy
Turbidity
Zeta potential
Hydrocarbons
Titration
Chain length
Rigidity
Electron microscopy
Differential scanning calorimetry
Electrostatics
Enthalpy
Atomic force microscopy

Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Roy, B., Guha, P., Nahak, P., Karmakar, G., Maiti, S., Mandal, A. K., ... Panda, A. K. (2018). Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer-Liposome Aggregates. ACS Omega, 3(9), 12235-12245. https://doi.org/10.1021/acsomega.8b01187
Roy, Biplab ; Guha, Pritam ; Nahak, Prasant ; Karmakar, Gourab ; Maiti, S. ; Mandal, A.K. ; Носков, Борис Анатольевич ; Быков, Алексей Геннадьевич ; Акентьев, Александр Владимирович ; Tsuchiya, K. ; Torigoe, K* ; Panda, Amiya Kumar. / Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer-Liposome Aggregates. In: ACS Omega. 2018 ; Vol. 3, No. 9. pp. 12235-12245.
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abstract = "Interaction between negatively charged liposomes and cationic polyamidoamine dendrimers of different generations was investigated through size, zeta potential, turbidity, electron microscopy, atomic force microscopy, fluorescence spectroscopy, and calorimetric studies. Liposomes with the binary combination of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) + dihexadecyl phosphate, DPPC + 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol, DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphate, and DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol were stable up to 60 days. The electrostatic nature of dendrimer-lipid bilayer interaction was evidenced through charge neutralization and subsequent reversal upon added dendrimer to liposome. Dendrimer-liposome interaction depended on its generation (5 > 4 > 3) in addition to the charge, head groups, and hydrocarbon chain length of lipids. Fluorescence anisotropy and differential scanning calorimetry studies suggest the fluidization of the bilayer, although the surface rigidity was enhanced by the added dendrimers. Thermodynamic parameters of the interaction processes were evaluated by isothermal titration and differential scanning calorimetric studies. The binding processes were exothermic in nature. The enthalpy of transition of the chain melting of lipids decreased systematically with increasing dendrimer concentration and generation. Dendrimer-liposome aggregates were nontoxic to healthy human blood cell, suggesting the potential of such aggregates as drug delivery systems.",
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Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer-Liposome Aggregates. / Roy, Biplab; Guha, Pritam; Nahak, Prasant; Karmakar, Gourab; Maiti, S.; Mandal, A.K.; Носков, Борис Анатольевич; Быков, Алексей Геннадьевич; Акентьев, Александр Владимирович; Tsuchiya, K.; Torigoe, K*; Panda, Amiya Kumar.

In: ACS Omega, Vol. 3, No. 9, 30.09.2018, p. 12235-12245.

Research output

TY - JOUR

T1 - Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer-Liposome Aggregates

AU - Roy, Biplab

AU - Guha, Pritam

AU - Nahak, Prasant

AU - Karmakar, Gourab

AU - Maiti, S.

AU - Mandal, A.K.

AU - Носков, Борис Анатольевич

AU - Быков, Алексей Геннадьевич

AU - Акентьев, Александр Владимирович

AU - Tsuchiya, K.

AU - Torigoe, K

AU - Panda, Amiya Kumar

PY - 2018/9/30

Y1 - 2018/9/30

N2 - Interaction between negatively charged liposomes and cationic polyamidoamine dendrimers of different generations was investigated through size, zeta potential, turbidity, electron microscopy, atomic force microscopy, fluorescence spectroscopy, and calorimetric studies. Liposomes with the binary combination of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) + dihexadecyl phosphate, DPPC + 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol, DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphate, and DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol were stable up to 60 days. The electrostatic nature of dendrimer-lipid bilayer interaction was evidenced through charge neutralization and subsequent reversal upon added dendrimer to liposome. Dendrimer-liposome interaction depended on its generation (5 > 4 > 3) in addition to the charge, head groups, and hydrocarbon chain length of lipids. Fluorescence anisotropy and differential scanning calorimetry studies suggest the fluidization of the bilayer, although the surface rigidity was enhanced by the added dendrimers. Thermodynamic parameters of the interaction processes were evaluated by isothermal titration and differential scanning calorimetric studies. The binding processes were exothermic in nature. The enthalpy of transition of the chain melting of lipids decreased systematically with increasing dendrimer concentration and generation. Dendrimer-liposome aggregates were nontoxic to healthy human blood cell, suggesting the potential of such aggregates as drug delivery systems.

AB - Interaction between negatively charged liposomes and cationic polyamidoamine dendrimers of different generations was investigated through size, zeta potential, turbidity, electron microscopy, atomic force microscopy, fluorescence spectroscopy, and calorimetric studies. Liposomes with the binary combination of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) + dihexadecyl phosphate, DPPC + 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol, DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphate, and DPPC + 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol were stable up to 60 days. The electrostatic nature of dendrimer-lipid bilayer interaction was evidenced through charge neutralization and subsequent reversal upon added dendrimer to liposome. Dendrimer-liposome interaction depended on its generation (5 > 4 > 3) in addition to the charge, head groups, and hydrocarbon chain length of lipids. Fluorescence anisotropy and differential scanning calorimetry studies suggest the fluidization of the bilayer, although the surface rigidity was enhanced by the added dendrimers. Thermodynamic parameters of the interaction processes were evaluated by isothermal titration and differential scanning calorimetric studies. The binding processes were exothermic in nature. The enthalpy of transition of the chain melting of lipids decreased systematically with increasing dendrimer concentration and generation. Dendrimer-liposome aggregates were nontoxic to healthy human blood cell, suggesting the potential of such aggregates as drug delivery systems.

KW - DESIGNING DENDRIMERS

KW - DETERGENT SOLUBILIZATION

KW - DRUG-DELIVERY

KW - LIPID-MEMBRANES

KW - MEMBRANE INTERACTIONS

KW - PHOSPHORUS DENDRIMERS

KW - PHYSICOCHEMICAL PROPERTIES

KW - POLYMERS

KW - TEMPERATURE

KW - VESICLES

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UR - http://pubs.acs.org/doi/10.1021/acsomega.8b01187

UR - http://www.mendeley.com/research/biophysical-correlates-composition-functionality-structure-dendrimerliposome-aggregates

U2 - 10.1021/acsomega.8b01187

DO - 10.1021/acsomega.8b01187

M3 - Article

VL - 3

SP - 12235

EP - 12245

JO - ACS Omega

JF - ACS Omega

SN - 2470-1343

IS - 9

ER -