Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications

Diego Pedreira de Oliveira, Tatiane Venturott Toniato, Ritchelli Ricci, Fernanda Roberta Marciano, Egor Prokofiev, Ruslan Z. Valiev, Anderson Oliveira Lobo, Alberto Moreira Jorge Júnior

Research output

Abstract

Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.

Original languageEnglish
Pages (from-to)1725-1736
Number of pages12
JournalInternational Journal of Nanomedicine
Volume14
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Cell adhesion
Titanium alloys
Equal channel angular pressing
Phosphatases
Cell membranes
Cytotoxicity
Processing
Nanostructured materials
Topography
Surface properties
Extrusion
Surface treatment
Etching
Plastic deformation
Bone
Porosity
Heat treatment
Tissue
Titanium
Proteins

Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

de Oliveira, Diego Pedreira ; Toniato, Tatiane Venturott ; Ricci, Ritchelli ; Marciano, Fernanda Roberta ; Prokofiev, Egor ; Valiev, Ruslan Z. ; Lobo, Anderson Oliveira ; Jorge Júnior, Alberto Moreira. / Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications. In: International Journal of Nanomedicine. 2019 ; Vol. 14. pp. 1725-1736.
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abstract = "Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.",
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Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications. / de Oliveira, Diego Pedreira; Toniato, Tatiane Venturott; Ricci, Ritchelli; Marciano, Fernanda Roberta; Prokofiev, Egor; Valiev, Ruslan Z.; Lobo, Anderson Oliveira; Jorge Júnior, Alberto Moreira.

In: International Journal of Nanomedicine, Vol. 14, 01.01.2019, p. 1725-1736.

Research output

TY - JOUR

T1 - Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications

AU - de Oliveira, Diego Pedreira

AU - Toniato, Tatiane Venturott

AU - Ricci, Ritchelli

AU - Marciano, Fernanda Roberta

AU - Prokofiev, Egor

AU - Valiev, Ruslan Z.

AU - Lobo, Anderson Oliveira

AU - Jorge Júnior, Alberto Moreira

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.

AB - Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.

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