The ability of DNA and DNP to bind actinomycin D (AMD) is often used at the present time to characterize the state of activity of the DNA in a deoxyribonucleoprotein (DNP). Free DNA binds more AMD than DNP, and the dependence of the degree of removal of the proteins from DNP on its capacity to bind AMD has been established. It has been shown that DNP from different sources differs in ability to bind AMD and that the matrix activity of the chromatin correlates to a certain degree with the amount of AMD bound. Therefore, the ability of the chromatin to bind AMD can to some degree characterize the free surface of the DNA matrix, a large part of which is covered by chromosomal proteins (mainly histones). Data obtained in a study of the binding of AMD with DNP isolated from nuclei of rabbit gastrocnemius muscles: intact, denervated, and subjected after denervation to indirect electrical stimulation, are presented.
|Number of pages||5|
|Journal||Journal of Evolutionary Biochemistry and Physiology|
|State||Published - 1 Jan 1975|
Scopus subject areas
- Ecology, Evolution, Behavior and Systematics