Basolateral pressure challenges mammary epithelial cell monolayer integrity, in vitro. doi: 10.1007/s10616-017-0130-3 Q2

Katharina S. Mießler, Constanze Vitzthum, Alexander G. Markov, Salah Amasheh

Research output

2 Citations (Scopus)

Abstract

Mammary gland epithelium is physiologically
exposed to variations of hydrostatic pressure
due to accumulation of milk and removal by suckling
and mechanical milking. Integrity of the mammary
gland epithelium primarily relies on the tight junction.
To analyze pressure-induced effects on the tight
junction, we established a modified Ussing chamber
and tested the hypothesis if hydrostatic pressure on the
basal side of the epithelium is able to affect barrier
properties in a mammary epithelial cell model,
in vitro. Therefore, a conventional Ussing chamber
was modified by an additional tube system to apply
hydrostatic pressure. Monolayers of the mammary
epithelial cell line HC11 were mounted in the modified
Ussing chambers and incubated with increasing basal
hydrostatic pressure. Transepithelial resistance and
short circuit current were recorded and compared to
controls. Hydrostatic pressure was stably applied and
incubation steps of 30 min were technically feasible,
leading to a decrease of transepithelial resistance and
an increase of short circuit current in all monolayers.
In a series of experiments simulating the physiological
exposure time by short intervals of 5 min, these
electrophysiological findings were also observed, and
monolayer integrity was not significantly perturbed as
analyzed by fluorescence immunohistochemistry
selectively staining tight junction proteins. Moreover,
electrophysiology demonstrated reversibility of
effects. In conclusion, the modified Ussing chamber
is an adequate method to analyze the effects of
hydrostatic pressure on epithelial cell monolayers,
in vitro. Both, the reduction of transepithelial resistance
and the increase of short circuit current may be
interpreted as protective reactions
Original languageEnglish
Article number10.1007/s10616-017-0130-3
Pages (from-to)567-576
Number of pages10
JournalCytotechnology
Volume70
Issue number2
Early online date29 Aug 2017
DOIs
Publication statusPublished - 1 Apr 2018

Scopus subject areas

  • Agricultural and Biological Sciences(all)

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