Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis

Natalia M. Yudintceva, Irina O. Bogolyubova, Alexandr N. Muraviov, Magomed G. Sheykhov, Tatiana I. Vinogradova, Evgenii G. Sokolovich, Igor A. Samusenko, Maxim A. Shevtsov

Research output

5 Citations (Scopus)

Abstract

Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.

Original languageEnglish
Pages (from-to)e1580-e1593
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Fingerprint

Cell- and Tissue-Based Therapy
Stem cells
Mesenchymal Stromal Cells
Urinary Bladder
Tuberculosis
Nanoparticles
Iron oxides
Urogenital Tuberculosis
Confocal microscopy
Cytotoxicity
Restoration
Cystectomy
Assays
Bone
Animals
Tissue Distribution
Confocal Microscopy
Tissue
Regeneration
Cell Survival

Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Yudintceva, N. M., Bogolyubova, I. O., Muraviov, A. N., Sheykhov, M. G., Vinogradova, T. I., Sokolovich, E. G., ... Shevtsov, M. A. (2018). Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis. Journal of Tissue Engineering and Regenerative Medicine, 12(3), e1580-e1593. https://doi.org/10.1002/term.2583
Yudintceva, Natalia M. ; Bogolyubova, Irina O. ; Muraviov, Alexandr N. ; Sheykhov, Magomed G. ; Vinogradova, Tatiana I. ; Sokolovich, Evgenii G. ; Samusenko, Igor A. ; Shevtsov, Maxim A. / Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis. In: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Vol. 12, No. 3. pp. e1580-e1593.
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abstract = "Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.",
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Yudintceva, NM, Bogolyubova, IO, Muraviov, AN, Sheykhov, MG, Vinogradova, TI, Sokolovich, EG, Samusenko, IA & Shevtsov, MA 2018, 'Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis', Journal of Tissue Engineering and Regenerative Medicine, vol. 12, no. 3, pp. e1580-e1593. https://doi.org/10.1002/term.2583

Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis. / Yudintceva, Natalia M.; Bogolyubova, Irina O.; Muraviov, Alexandr N.; Sheykhov, Magomed G.; Vinogradova, Tatiana I.; Sokolovich, Evgenii G.; Samusenko, Igor A.; Shevtsov, Maxim A.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 3, 01.03.2018, p. e1580-e1593.

Research output

TY - JOUR

T1 - Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis

AU - Yudintceva, Natalia M.

AU - Bogolyubova, Irina O.

AU - Muraviov, Alexandr N.

AU - Sheykhov, Magomed G.

AU - Vinogradova, Tatiana I.

AU - Sokolovich, Evgenii G.

AU - Samusenko, Igor A.

AU - Shevtsov, Maxim A.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.

AB - Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.

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