Application of spectral methods for studying DNA damage induced by gamma-radiation

Svetlana A. Tankovskaia, Omar M. Kotb, Olga A. Dommes, Sofia V. Paston

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Spectral methods can provide a variety of possibilities to determine several types of radiation-induced DNA damage, such as nucleobase destruction and local denaturation. DNA UV absorption and CD spectra measured at room temperature undergo noticeable alteration under the action of γ-radiation. We have applied the Spirin method of total nucleobases determination, and have measured the molar extinction coefficient of DNA and DNA CD spectra for solutions with different NaCl concentrations (3 mM–3.2 M) and containing MgCl 2, exposed to γ-radiation with the doses of 0–10 3 Gy. The melting temperatures of DNA in irradiated solutions at the doses of 0–50 Gy were obtained with the help of spectrophotometric melting. It was found that the amount of destructed nucleobases and radiation-induced loss of DNA helicity significantly decreases with the rise of the ionic strength of the irradiated solution. Substitution of a portion of Na + ions on Mg 2+ while keeping the total ionic strength constant (μ = 5 mM) does not affect the considered radiation effects. The role of the structure and composition of the DNA secondary hydration layer in the radiation-induced damages is discussed.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume200
Early online date10 Apr 2018
DOIs
StatePublished - 5 Jul 2018

Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biophysics
  • Analytical Chemistry
  • Instrumentation
  • Spectroscopy

Keywords

  • Circular dichroism
  • DNA damage
  • DNA hydration
  • DNA melting
  • Ionizing radiation
  • Ions
  • Nucleobases destruction
  • UV absorption spectroscopy
  • HYDRATED DNA
  • NUCLEIC-ACID
  • RAMAN-SPECTROSCOPY
  • CIRCULAR-DICHROISM
  • CONFORMATIONAL-CHANGES
  • CELLULAR-DNA
  • PLASMID DNA
  • IONIC-STRENGTH
  • METAL-IONS
  • Nudeobases destruction
  • REAL-TIME

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