Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test

Research outputpeer-review

Abstract

Schizophrenia is an extremely debilitating psychiatric disorder. Levomepromazine is a neuroleptic drug that can be used for manic phases of bipolar disorder, and is also widely used in palliative care. However, little is known about its efficacy for schizophrenia. Mounting evidence suggests the use of zebrafish in antipsychotic drugs screening due to its high genetic and physiological homology to humans. Here we apply zebrafish novel tank test to study the effects of levomepromazine on their locomotion and behavior. Behavioral testing was performed in adult wild-type outbredshort-fin zebrafish between 11.00 and 17.00 h, using tanks (20x20x5 cm) with water adjusted to the holding room temperature, to assess zebrafish behavior. Prior to testing, fish were preexposed in a 0.5-L plastic beaker for 20 min to either drug-treated or drug-free water. For experiment, fish were randomly divided into 3 groups (n=9-10): drug-free control and fish preexposed to 25 mg/L or 50 mg/L of levomepromazine. Doses were chosen based on pilot studies in which higher (100 mg/L) resulted in ataxic responses. Zebrafish behavior was recorded on webcam and then processed in Noldus EthoVision XT 11.5. Was studied such behavioral endpoints as distance moved (cm), moving and not moving durations (s), time spent in the top part of tank (s) and number of transitions from bottom to top (n), absolute mean meandering (deg/cm). For statistical evaluation was used Kruskal-Wallis test with posthoc Dunn’s test for pair comparisons of statistically significant KW data. Data is represented as Mean±SEM. Overall, levomepromazine exposure alters zebrafish behavior, decreasing time spent moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 253.590±12.5572s, 196,462±21.7011s and 158.096±32.158 respectively), increasing time spent not moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 43.833±12.4763, 102.192±21.8682, 135.638±29.340 respectively), increasing absolute mean meandering (p<0.01 for KW comparison, p>0.05 for 25 mg/L and p<0.01 for 50 mg/L in Dunn’s test vs. control; 305.994±126.6485, 1177.727±380.0390, 7373.960±2977.579 respectively) and decreasing number of zone transitions (p<0.001 for KW comparison, p<0.001 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 18.333±3.1091, 2.700±0.8699, 5.800±2.107 respectively). There was no difference in distance moved and time spent in the top half of tank (p>0.05). Taken together, this data suggests a mild hypolocomotor profile. Interestingly, giving changes in zone transitions and time spent in top that occurred in different directions compared to control, lower doses of the drug also seem to produce anxiolytic effect, which becomes anxiogenic in higher dose (182.8054±11.44136, 227.0210±22.62603, 136.9333±35,42235 for control, 25 and 50 mg/L doses.
Original languageEnglish
Title of host publicationАктуальные проблемы трансляционной биомедицины - 2018
Subtitle of host publicationСборник тезисов
Place of PublicationСПб.
PublisherИздательство Санкт-Петербургского университета
Pages69
Publication statusPublished - 20 Jul 2018
Event4-я ежегодная конференция Института Трансляционной
биомедицины СПбГУ (ИТБМ СПбГУ) «Актуальные проблемы трансляционной биомедицины - 2018»
- Санкт-Петербург
Duration: 20 Jul 201822 Jul 2018

Conference

Conference4-я ежегодная конференция Института Трансляционной
биомедицины СПбГУ (ИТБМ СПбГУ) «Актуальные проблемы трансляционной биомедицины - 2018»
CountryRussian Federation
CityСанкт-Петербург
Period20/07/1822/07/18

Fingerprint

Methotrimeprazine
Zebrafish
Antipsychotic Agents
Fishes
Schizophrenia
Pharmaceutical Preparations
Preclinical Drug Evaluations
Water
Drug and Narcotic Control
Anti-Anxiety Agents
Locomotion
Palliative Care
Bipolar Disorder
Plastics
Psychiatry
Temperature

Cite this

Demin, K. A., & Kalueff, A. V. (2018). Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test. In Актуальные проблемы трансляционной биомедицины - 2018: Сборник тезисов (pp. 69). СПб.: Издательство Санкт-Петербургского университета.
Demin, K.A. ; Kalueff, A.V. / Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test. Актуальные проблемы трансляционной биомедицины - 2018: Сборник тезисов. СПб. : Издательство Санкт-Петербургского университета, 2018. pp. 69
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abstract = "Schizophrenia is an extremely debilitating psychiatric disorder. Levomepromazine is a neuroleptic drug that can be used for manic phases of bipolar disorder, and is also widely used in palliative care. However, little is known about its efficacy for schizophrenia. Mounting evidence suggests the use of zebrafish in antipsychotic drugs screening due to its high genetic and physiological homology to humans. Here we apply zebrafish novel tank test to study the effects of levomepromazine on their locomotion and behavior. Behavioral testing was performed in adult wild-type outbredshort-fin zebrafish between 11.00 and 17.00 h, using tanks (20x20x5 cm) with water adjusted to the holding room temperature, to assess zebrafish behavior. Prior to testing, fish were preexposed in a 0.5-L plastic beaker for 20 min to either drug-treated or drug-free water. For experiment, fish were randomly divided into 3 groups (n=9-10): drug-free control and fish preexposed to 25 mg/L or 50 mg/L of levomepromazine. Doses were chosen based on pilot studies in which higher (100 mg/L) resulted in ataxic responses. Zebrafish behavior was recorded on webcam and then processed in Noldus EthoVision XT 11.5. Was studied such behavioral endpoints as distance moved (cm), moving and not moving durations (s), time spent in the top part of tank (s) and number of transitions from bottom to top (n), absolute mean meandering (deg/cm). For statistical evaluation was used Kruskal-Wallis test with posthoc Dunn’s test for pair comparisons of statistically significant KW data. Data is represented as Mean±SEM. Overall, levomepromazine exposure alters zebrafish behavior, decreasing time spent moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 253.590±12.5572s, 196,462±21.7011s and 158.096±32.158 respectively), increasing time spent not moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 43.833±12.4763, 102.192±21.8682, 135.638±29.340 respectively), increasing absolute mean meandering (p<0.01 for KW comparison, p>0.05 for 25 mg/L and p<0.01 for 50 mg/L in Dunn’s test vs. control; 305.994±126.6485, 1177.727±380.0390, 7373.960±2977.579 respectively) and decreasing number of zone transitions (p<0.001 for KW comparison, p<0.001 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 18.333±3.1091, 2.700±0.8699, 5.800±2.107 respectively). There was no difference in distance moved and time spent in the top half of tank (p>0.05). Taken together, this data suggests a mild hypolocomotor profile. Interestingly, giving changes in zone transitions and time spent in top that occurred in different directions compared to control, lower doses of the drug also seem to produce anxiolytic effect, which becomes anxiogenic in higher dose (182.8054±11.44136, 227.0210±22.62603, 136.9333±35,42235 for control, 25 and 50 mg/L doses.",
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Demin, KA & Kalueff, AV 2018, Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test. in Актуальные проблемы трансляционной биомедицины - 2018: Сборник тезисов. Издательство Санкт-Петербургского университета, СПб., pp. 69, Санкт-Петербург, 20/07/18.

Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test. / Demin, K.A.; Kalueff, A.V.

Актуальные проблемы трансляционной биомедицины - 2018: Сборник тезисов. СПб. : Издательство Санкт-Петербургского университета, 2018. p. 69.

Research outputpeer-review

TY - CHAP

T1 - Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test

AU - Demin, K.A.

AU - Kalueff, A.V.

PY - 2018/7/20

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N2 - Schizophrenia is an extremely debilitating psychiatric disorder. Levomepromazine is a neuroleptic drug that can be used for manic phases of bipolar disorder, and is also widely used in palliative care. However, little is known about its efficacy for schizophrenia. Mounting evidence suggests the use of zebrafish in antipsychotic drugs screening due to its high genetic and physiological homology to humans. Here we apply zebrafish novel tank test to study the effects of levomepromazine on their locomotion and behavior. Behavioral testing was performed in adult wild-type outbredshort-fin zebrafish between 11.00 and 17.00 h, using tanks (20x20x5 cm) with water adjusted to the holding room temperature, to assess zebrafish behavior. Prior to testing, fish were preexposed in a 0.5-L plastic beaker for 20 min to either drug-treated or drug-free water. For experiment, fish were randomly divided into 3 groups (n=9-10): drug-free control and fish preexposed to 25 mg/L or 50 mg/L of levomepromazine. Doses were chosen based on pilot studies in which higher (100 mg/L) resulted in ataxic responses. Zebrafish behavior was recorded on webcam and then processed in Noldus EthoVision XT 11.5. Was studied such behavioral endpoints as distance moved (cm), moving and not moving durations (s), time spent in the top part of tank (s) and number of transitions from bottom to top (n), absolute mean meandering (deg/cm). For statistical evaluation was used Kruskal-Wallis test with posthoc Dunn’s test for pair comparisons of statistically significant KW data. Data is represented as Mean±SEM. Overall, levomepromazine exposure alters zebrafish behavior, decreasing time spent moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 253.590±12.5572s, 196,462±21.7011s and 158.096±32.158 respectively), increasing time spent not moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 43.833±12.4763, 102.192±21.8682, 135.638±29.340 respectively), increasing absolute mean meandering (p<0.01 for KW comparison, p>0.05 for 25 mg/L and p<0.01 for 50 mg/L in Dunn’s test vs. control; 305.994±126.6485, 1177.727±380.0390, 7373.960±2977.579 respectively) and decreasing number of zone transitions (p<0.001 for KW comparison, p<0.001 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 18.333±3.1091, 2.700±0.8699, 5.800±2.107 respectively). There was no difference in distance moved and time spent in the top half of tank (p>0.05). Taken together, this data suggests a mild hypolocomotor profile. Interestingly, giving changes in zone transitions and time spent in top that occurred in different directions compared to control, lower doses of the drug also seem to produce anxiolytic effect, which becomes anxiogenic in higher dose (182.8054±11.44136, 227.0210±22.62603, 136.9333±35,42235 for control, 25 and 50 mg/L doses.

AB - Schizophrenia is an extremely debilitating psychiatric disorder. Levomepromazine is a neuroleptic drug that can be used for manic phases of bipolar disorder, and is also widely used in palliative care. However, little is known about its efficacy for schizophrenia. Mounting evidence suggests the use of zebrafish in antipsychotic drugs screening due to its high genetic and physiological homology to humans. Here we apply zebrafish novel tank test to study the effects of levomepromazine on their locomotion and behavior. Behavioral testing was performed in adult wild-type outbredshort-fin zebrafish between 11.00 and 17.00 h, using tanks (20x20x5 cm) with water adjusted to the holding room temperature, to assess zebrafish behavior. Prior to testing, fish were preexposed in a 0.5-L plastic beaker for 20 min to either drug-treated or drug-free water. For experiment, fish were randomly divided into 3 groups (n=9-10): drug-free control and fish preexposed to 25 mg/L or 50 mg/L of levomepromazine. Doses were chosen based on pilot studies in which higher (100 mg/L) resulted in ataxic responses. Zebrafish behavior was recorded on webcam and then processed in Noldus EthoVision XT 11.5. Was studied such behavioral endpoints as distance moved (cm), moving and not moving durations (s), time spent in the top part of tank (s) and number of transitions from bottom to top (n), absolute mean meandering (deg/cm). For statistical evaluation was used Kruskal-Wallis test with posthoc Dunn’s test for pair comparisons of statistically significant KW data. Data is represented as Mean±SEM. Overall, levomepromazine exposure alters zebrafish behavior, decreasing time spent moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 253.590±12.5572s, 196,462±21.7011s and 158.096±32.158 respectively), increasing time spent not moving (p<0.05 for KW comparison, p>0.05 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 43.833±12.4763, 102.192±21.8682, 135.638±29.340 respectively), increasing absolute mean meandering (p<0.01 for KW comparison, p>0.05 for 25 mg/L and p<0.01 for 50 mg/L in Dunn’s test vs. control; 305.994±126.6485, 1177.727±380.0390, 7373.960±2977.579 respectively) and decreasing number of zone transitions (p<0.001 for KW comparison, p<0.001 for 25 mg/L and p<0.05 for 50 mg/L in Dunn’s test vs. control; 18.333±3.1091, 2.700±0.8699, 5.800±2.107 respectively). There was no difference in distance moved and time spent in the top half of tank (p>0.05). Taken together, this data suggests a mild hypolocomotor profile. Interestingly, giving changes in zone transitions and time spent in top that occurred in different directions compared to control, lower doses of the drug also seem to produce anxiolytic effect, which becomes anxiogenic in higher dose (182.8054±11.44136, 227.0210±22.62603, 136.9333±35,42235 for control, 25 and 50 mg/L doses.

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PB - Издательство Санкт-Петербургского университета

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Demin KA, Kalueff AV. Antipsychotic drug levomepromazine action on adult zebrafish behavior in novel tank test. In Актуальные проблемы трансляционной биомедицины - 2018: Сборник тезисов. СПб.: Издательство Санкт-Петербургского университета. 2018. p. 69