An Integrated Approach for Monitoring Cell Senescence. Cell senescence. Chapter 26

Tatiana V. Pospelova, Zhanna V. Chitikova, Valery A. Pospelov

Research output

15 Citations (Scopus)

Abstract

Cellular senescence is considered as a crucial mechanism of tumor suppression that helps to prevent the growth of cells at risk for neoplastic transformation. In normal cells, cellular senescence induces an irreversible cell cycle arrest in response to telomere dysfunction, oncogene activation, genotoxic stress and a persistent DNA damage response (DDR). This process is accompanied by dramatic changes in cell morphology as well as in the activity of several signaling pathways. The senescent phenotype is multifaceted. In addition to an obligatory proliferation arrest, senescent cells manifest various senescence markers: mTOR-mediated hypertrophic growth (cell size increase), cell fl attening, senescence-associated β galactosidase (SA- β gal) staining, expression of negative cell cycle regulators p53, p21 Waf1 and p16 Ink4a , speci fi c chromatin reorganization including DNA segments with chromatin alterations reinforcing senescence (DNA-SCARS), senescence- associated secretory phenotype (SASP) and other featur
Original languageEnglish
Title of host publicationMethods in Molecular Biology. Methods and Protocols
PublisherHumana Press
Pages538, 383-408
ISBN (Print)978-1-62703-238-4
DOIs
Publication statusPublished - 2013
Externally publishedYes

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