An Approach to Better Estimation of the Initial Velocity of Enzyme Reactions

N. M. Malygina, T. A. Petrova, A. Y. Lianguzov, A. M. Ivanov

Research output

Abstract

A new algorithm for computation of the initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce the systematic error of measurements to the minimum, to estimate the reaction velocity in test samples regardless of the enzyme activity levels, and to minimize the assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag phase in the kinetic curve.

Original languageEnglish
Pages (from-to)311–312
JournalDoklady Biochemistry and Biophysics
Volume488
Issue number1
Early online date25 Nov 2019
Publication statusPublished - 2019

Fingerprint

Enzymes
Salivary alpha-Amylases
Systematic errors
Enzyme activity
Assays
Kinetics

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Malygina, N. M. ; Petrova, T. A. ; Lianguzov, A. Y. ; Ivanov, A. M. . / An Approach to Better Estimation of the Initial Velocity of Enzyme Reactions. In: Doklady Biochemistry and Biophysics. 2019 ; Vol. 488, No. 1. pp. 311–312.
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Malygina, NM, Petrova, TA, Lianguzov, AY & Ivanov, AM 2019, 'An Approach to Better Estimation of the Initial Velocity of Enzyme Reactions', Doklady Biochemistry and Biophysics, vol. 488, no. 1, pp. 311–312.

An Approach to Better Estimation of the Initial Velocity of Enzyme Reactions. / Malygina, N. M. ; Petrova, T. A. ; Lianguzov, A. Y. ; Ivanov, A. M. .

In: Doklady Biochemistry and Biophysics, Vol. 488, No. 1, 2019, p. 311–312.

Research output

TY - JOUR

T1 - An Approach to Better Estimation of the Initial Velocity of Enzyme Reactions

AU - Malygina, N. M.

AU - Petrova, T. A.

AU - Lianguzov, A. Y.

AU - Ivanov, A. M.

N1 - Malygina, N.M., Petrova, T.A., Lianguzov, A.Y. et al. Dokl Biochem Biophys (2019) 488: 311. https://doi.org/10.1134/S1607672919050053

PY - 2019

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AB - A new algorithm for computation of the initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce the systematic error of measurements to the minimum, to estimate the reaction velocity in test samples regardless of the enzyme activity levels, and to minimize the assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag phase in the kinetic curve.

KW - enzyme kinetics, initial reaction velocity, salivary alpha-amylase

UR - https://link.springer.com/article/10.1134/S1607672919050053

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