Agrobacterial tumors interfere with nodulation and demonstrate the expression of nodulation-induced CLE genes in pea

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Abstract

CLAVATA (CLV) system including CLV1-like kinase and CLE-peptides is the part of the AON (autoregulation of nodulation) that controls nodule number in legume plants. Moreover, CLV system plays a key role in meristems, where it regulates the expression of WOX genes in organizing centers. Recently, we found that WOX5 homolog in pea is also expressed in nodules and in tumors induced by Agrobacterium tumefaciens. Based on this, we hypothesized that both nodules and agrobacterial tumors may be regulated by and may trigger the same components of AON, including the same WOX and CLV genes. Here, we found that pea plants with agrobacterial tumors induced prior to rhizobial inoculation had reduced the number of nodules. This effect was absent in pea sym29 mutant defective in CLV1-like kinase, the key component of AON. That suggests that agrobacterial tumors may produce a signal activating CLV1-like kinase and thereby decrease the nodule number. Since CLE peptides are known to act upstream of CLV1-like kinase, expression analysis of CLE genes has been performed both in developing nodules and tumors. Overall, 45 CLE genes were identified, and among them nine nodulation-induced CLEs were found in pea. In agrobacterial tumors, no expression of nodule-specific CLE genes the homologues of which inhibit nodulation in other legumes was observed. However, increased expression of two other nodulation-induced CLE genes was observed in agrobacterial tumors, suggesting that CLE genes are expressed in tumors that may still contribute to autoregulatory processes suppressing nodulation.

Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalJournal of Plant Physiology
Volume221
DOIs
Publication statusPublished - 2018

Fingerprint

Peas
nodulation
peas
neoplasms
autoregulation
Genes
Neoplasms
phosphotransferases (kinases)
Phosphotransferases
genes
Homeostasis
Fabaceae
legumes
peptides
Agrobacterium tumefaciens
Peptides
Meristem
Agrobacterium radiobacter
meristems
Gene Expression

Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{77c3abebfe4a4b1aa9bf7ed2b31b9736,
title = "Agrobacterial tumors interfere with nodulation and demonstrate the expression of nodulation-induced CLE genes in pea",
abstract = "CLAVATA (CLV) system including CLV1-like kinase and CLE-peptides is the part of the AON (autoregulation of nodulation) that controls nodule number in legume plants. Moreover, CLV system plays a key role in meristems, where it regulates the expression of WOX genes in organizing centers. Recently, we found that WOX5 homolog in pea is also expressed in nodules and in tumors induced by Agrobacterium tumefaciens. Based on this, we hypothesized that both nodules and agrobacterial tumors may be regulated by and may trigger the same components of AON, including the same WOX and CLV genes. Here, we found that pea plants with agrobacterial tumors induced prior to rhizobial inoculation had reduced the number of nodules. This effect was absent in pea sym29 mutant defective in CLV1-like kinase, the key component of AON. That suggests that agrobacterial tumors may produce a signal activating CLV1-like kinase and thereby decrease the nodule number. Since CLE peptides are known to act upstream of CLV1-like kinase, expression analysis of CLE genes has been performed both in developing nodules and tumors. Overall, 45 CLE genes were identified, and among them nine nodulation-induced CLEs were found in pea. In agrobacterial tumors, no expression of nodule-specific CLE genes the homologues of which inhibit nodulation in other legumes was observed. However, increased expression of two other nodulation-induced CLE genes was observed in agrobacterial tumors, suggesting that CLE genes are expressed in tumors that may still contribute to autoregulatory processes suppressing nodulation.",
keywords = "Agrobacterial tumors, Autoregulation of nodulation, CLE, CLV1-like kinase, Nodule development, WOX-CLV",
author = "Samorodova, {A P} and Tvorogova, {V E} and Tkachenko, {A A} and Potsenkovskaya, {E A} and Lebedeva, {М А} and Tikhonovich, {I A} and Lutova, {L А}",
note = "Copyright {\circledC} 2017 Elsevier GmbH. All rights reserved.",
year = "2018",
doi = "10.1016/j.jplph.2017.12.005",
language = "English",
volume = "221",
pages = "94--100",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Elsevier",

}

TY - JOUR

T1 - Agrobacterial tumors interfere with nodulation and demonstrate the expression of nodulation-induced CLE genes in pea

AU - Samorodova, A P

AU - Tvorogova, V E

AU - Tkachenko, A A

AU - Potsenkovskaya, E A

AU - Lebedeva, М А

AU - Tikhonovich, I A

AU - Lutova, L А

N1 - Copyright © 2017 Elsevier GmbH. All rights reserved.

PY - 2018

Y1 - 2018

N2 - CLAVATA (CLV) system including CLV1-like kinase and CLE-peptides is the part of the AON (autoregulation of nodulation) that controls nodule number in legume plants. Moreover, CLV system plays a key role in meristems, where it regulates the expression of WOX genes in organizing centers. Recently, we found that WOX5 homolog in pea is also expressed in nodules and in tumors induced by Agrobacterium tumefaciens. Based on this, we hypothesized that both nodules and agrobacterial tumors may be regulated by and may trigger the same components of AON, including the same WOX and CLV genes. Here, we found that pea plants with agrobacterial tumors induced prior to rhizobial inoculation had reduced the number of nodules. This effect was absent in pea sym29 mutant defective in CLV1-like kinase, the key component of AON. That suggests that agrobacterial tumors may produce a signal activating CLV1-like kinase and thereby decrease the nodule number. Since CLE peptides are known to act upstream of CLV1-like kinase, expression analysis of CLE genes has been performed both in developing nodules and tumors. Overall, 45 CLE genes were identified, and among them nine nodulation-induced CLEs were found in pea. In agrobacterial tumors, no expression of nodule-specific CLE genes the homologues of which inhibit nodulation in other legumes was observed. However, increased expression of two other nodulation-induced CLE genes was observed in agrobacterial tumors, suggesting that CLE genes are expressed in tumors that may still contribute to autoregulatory processes suppressing nodulation.

AB - CLAVATA (CLV) system including CLV1-like kinase and CLE-peptides is the part of the AON (autoregulation of nodulation) that controls nodule number in legume plants. Moreover, CLV system plays a key role in meristems, where it regulates the expression of WOX genes in organizing centers. Recently, we found that WOX5 homolog in pea is also expressed in nodules and in tumors induced by Agrobacterium tumefaciens. Based on this, we hypothesized that both nodules and agrobacterial tumors may be regulated by and may trigger the same components of AON, including the same WOX and CLV genes. Here, we found that pea plants with agrobacterial tumors induced prior to rhizobial inoculation had reduced the number of nodules. This effect was absent in pea sym29 mutant defective in CLV1-like kinase, the key component of AON. That suggests that agrobacterial tumors may produce a signal activating CLV1-like kinase and thereby decrease the nodule number. Since CLE peptides are known to act upstream of CLV1-like kinase, expression analysis of CLE genes has been performed both in developing nodules and tumors. Overall, 45 CLE genes were identified, and among them nine nodulation-induced CLEs were found in pea. In agrobacterial tumors, no expression of nodule-specific CLE genes the homologues of which inhibit nodulation in other legumes was observed. However, increased expression of two other nodulation-induced CLE genes was observed in agrobacterial tumors, suggesting that CLE genes are expressed in tumors that may still contribute to autoregulatory processes suppressing nodulation.

KW - Agrobacterial tumors

KW - Autoregulation of nodulation

KW - CLE

KW - CLV1-like kinase

KW - Nodule development

KW - WOX-CLV

UR - http://www.scopus.com/inward/record.url?scp=85038263590&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2017.12.005

DO - 10.1016/j.jplph.2017.12.005

M3 - Article

VL - 221

SP - 94

EP - 100

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

ER -