Age-Dependent Electrocorticogram Dynamics and Epileptogenic Responsiveness in Rats Subjected to Prenatal Hypoxia

D.S. Kalinina , D.S. Vasilev , A.B. Volnova , N.N. Nalivaeva , I.A. Zhuravin

Research output

Abstract

Using electrocorticogram (ECoG) analysis, we compared age-related dynamics of general neuronal activity and convulsive epileptiform responsiveness induced by intracortical microinjections of 4-aminopyridine (4-AP) in control Wistar rats and those subjected to prenatal hypoxia (Hx; E14; 7% O 2, 3 h). The studies were carried out in three age periods roughly corresponding to childhood (P20-27), adolescence (P30-45), and adulthood (P90-120). It was found that in the process of postnatal development of the control rats, the peak of the ECoG power spectrum density (PSD) of the theta rhythm during wakefulness shifted from the low to the higher frequency, while in the Hx rats this shift had the opposite direction. Moreover, the Hx rats had different frequency characteristics of the ECoG PSD and longer episodes of spike-and-wave discharges caused by 4-AP injections compared to the controls. The total ECoG PSD of slow-wave sleep (1-5 Hz) was also dramatically decreased in the process of development of the Hx rats. Such alterations in PSD could be explained by the changes in balance of the excitation and inhibition processes in the cortical networks. Analyzing protein levels of neurotransmitter transporters in the brain structures of the Hx rats, we found that the content of the glutamate transporter EAAT1 was higher in the parietal cortex in all age groups of Hx rats while in the hippocampus it decreased during postnatal development compared to controls. Furthermore, the content of the vesicular acetylcholine transporter in the parietal cortex, and of the inhibitory GABA transporter 1 in the hippocampus, was also affected by prenatal Hx. These data suggest that prenatal Hx results in a shift in the excitatory and inhibitory balance in the rat cortex towards excitation, making the rat's brain more vulnerable to the effects of proconvulsant drugs and predisposing animals to epileptogenesis during postnatal life.

Original languageEnglish
Pages (from-to)56-66
Number of pages11
JournalDevelopmental Neuroscience
Volume41
Issue number1-2
DOIs
Publication statusPublished - Sep 2019

Fingerprint

4-Aminopyridine
Parietal Lobe
Hippocampus
Neurotransmitter Transport Proteins
Vesicular Acetylcholine Transport Proteins
GABA Plasma Membrane Transport Proteins
Theta Rhythm
Amino Acid Transport System X-AG
Hypoxia
Wakefulness
Brain
Microinjections
Wistar Rats
Sleep
Age Groups
Injections
Pharmaceutical Preparations
Inhibition (Psychology)
Direction compound

Scopus subject areas

  • Neuroscience(all)
  • Neurology
  • Developmental Neuroscience

Cite this

@article{3d09439ee841423bb453f4aa37bd4122,
title = "Age-Dependent Electrocorticogram Dynamics and Epileptogenic Responsiveness in Rats Subjected to Prenatal Hypoxia",
abstract = "Using electrocorticogram (ECoG) analysis, we compared age-related dynamics of general neuronal activity and convulsive epileptiform responsiveness induced by intracortical microinjections of 4-aminopyridine (4-AP) in control Wistar rats and those subjected to prenatal hypoxia (Hx; E14; 7{\%} O 2, 3 h). The studies were carried out in three age periods roughly corresponding to childhood (P20-27), adolescence (P30-45), and adulthood (P90-120). It was found that in the process of postnatal development of the control rats, the peak of the ECoG power spectrum density (PSD) of the theta rhythm during wakefulness shifted from the low to the higher frequency, while in the Hx rats this shift had the opposite direction. Moreover, the Hx rats had different frequency characteristics of the ECoG PSD and longer episodes of spike-and-wave discharges caused by 4-AP injections compared to the controls. The total ECoG PSD of slow-wave sleep (1-5 Hz) was also dramatically decreased in the process of development of the Hx rats. Such alterations in PSD could be explained by the changes in balance of the excitation and inhibition processes in the cortical networks. Analyzing protein levels of neurotransmitter transporters in the brain structures of the Hx rats, we found that the content of the glutamate transporter EAAT1 was higher in the parietal cortex in all age groups of Hx rats while in the hippocampus it decreased during postnatal development compared to controls. Furthermore, the content of the vesicular acetylcholine transporter in the parietal cortex, and of the inhibitory GABA transporter 1 in the hippocampus, was also affected by prenatal Hx. These data suggest that prenatal Hx results in a shift in the excitatory and inhibitory balance in the rat cortex towards excitation, making the rat's brain more vulnerable to the effects of proconvulsant drugs and predisposing animals to epileptogenesis during postnatal life.",
keywords = "Electrocorticogram dynamicsEpilepsyPrenatal hypoxiaCortexHippocampusSpike-and-wave dischargesSlow-wave sleep4-Aminopyridine, 4-Aminopyridine, Cortex, Electrocorticogram dynamics, Epilepsy, Hippocampus, Prenatal hypoxia, Slow-wave sleep, Spike-and-wave discharges, TRANSPORTER, WHITE-MATTER, BRAIN-DEVELOPMENT, VULNERABILITY, ADOLESCENCE, 4-AMINOPYRIDINE, ORGANIZATION, STRUCTURAL-CHANGES, ONTOGENY, NERVOUS-TISSUE",
author = "D.S. Kalinina and D.S. Vasilev and A.B. Volnova and N.N. Nalivaeva and I.A. Zhuravin",
year = "2019",
month = "9",
doi = "10.1159/000497224",
language = "English",
volume = "41",
pages = "56--66",
journal = "Developmental Neuroscience",
issn = "0378-5866",
publisher = "S. Karger AG",
number = "1-2",

}

TY - JOUR

T1 - Age-Dependent Electrocorticogram Dynamics and Epileptogenic Responsiveness in Rats Subjected to Prenatal Hypoxia

AU - Kalinina , D.S.

AU - Vasilev , D.S.

AU - Volnova , A.B.

AU - Nalivaeva , N.N.

AU - Zhuravin , I.A.

PY - 2019/9

Y1 - 2019/9

N2 - Using electrocorticogram (ECoG) analysis, we compared age-related dynamics of general neuronal activity and convulsive epileptiform responsiveness induced by intracortical microinjections of 4-aminopyridine (4-AP) in control Wistar rats and those subjected to prenatal hypoxia (Hx; E14; 7% O 2, 3 h). The studies were carried out in three age periods roughly corresponding to childhood (P20-27), adolescence (P30-45), and adulthood (P90-120). It was found that in the process of postnatal development of the control rats, the peak of the ECoG power spectrum density (PSD) of the theta rhythm during wakefulness shifted from the low to the higher frequency, while in the Hx rats this shift had the opposite direction. Moreover, the Hx rats had different frequency characteristics of the ECoG PSD and longer episodes of spike-and-wave discharges caused by 4-AP injections compared to the controls. The total ECoG PSD of slow-wave sleep (1-5 Hz) was also dramatically decreased in the process of development of the Hx rats. Such alterations in PSD could be explained by the changes in balance of the excitation and inhibition processes in the cortical networks. Analyzing protein levels of neurotransmitter transporters in the brain structures of the Hx rats, we found that the content of the glutamate transporter EAAT1 was higher in the parietal cortex in all age groups of Hx rats while in the hippocampus it decreased during postnatal development compared to controls. Furthermore, the content of the vesicular acetylcholine transporter in the parietal cortex, and of the inhibitory GABA transporter 1 in the hippocampus, was also affected by prenatal Hx. These data suggest that prenatal Hx results in a shift in the excitatory and inhibitory balance in the rat cortex towards excitation, making the rat's brain more vulnerable to the effects of proconvulsant drugs and predisposing animals to epileptogenesis during postnatal life.

AB - Using electrocorticogram (ECoG) analysis, we compared age-related dynamics of general neuronal activity and convulsive epileptiform responsiveness induced by intracortical microinjections of 4-aminopyridine (4-AP) in control Wistar rats and those subjected to prenatal hypoxia (Hx; E14; 7% O 2, 3 h). The studies were carried out in three age periods roughly corresponding to childhood (P20-27), adolescence (P30-45), and adulthood (P90-120). It was found that in the process of postnatal development of the control rats, the peak of the ECoG power spectrum density (PSD) of the theta rhythm during wakefulness shifted from the low to the higher frequency, while in the Hx rats this shift had the opposite direction. Moreover, the Hx rats had different frequency characteristics of the ECoG PSD and longer episodes of spike-and-wave discharges caused by 4-AP injections compared to the controls. The total ECoG PSD of slow-wave sleep (1-5 Hz) was also dramatically decreased in the process of development of the Hx rats. Such alterations in PSD could be explained by the changes in balance of the excitation and inhibition processes in the cortical networks. Analyzing protein levels of neurotransmitter transporters in the brain structures of the Hx rats, we found that the content of the glutamate transporter EAAT1 was higher in the parietal cortex in all age groups of Hx rats while in the hippocampus it decreased during postnatal development compared to controls. Furthermore, the content of the vesicular acetylcholine transporter in the parietal cortex, and of the inhibitory GABA transporter 1 in the hippocampus, was also affected by prenatal Hx. These data suggest that prenatal Hx results in a shift in the excitatory and inhibitory balance in the rat cortex towards excitation, making the rat's brain more vulnerable to the effects of proconvulsant drugs and predisposing animals to epileptogenesis during postnatal life.

KW - Electrocorticogram dynamicsEpilepsyPrenatal hypoxiaCortexHippocampusSpike-and-wave dischargesSlow-wave sleep4-Aminopyridine

KW - 4-Aminopyridine

KW - Cortex

KW - Electrocorticogram dynamics

KW - Epilepsy

KW - Hippocampus

KW - Prenatal hypoxia

KW - Slow-wave sleep

KW - Spike-and-wave discharges

KW - TRANSPORTER

KW - WHITE-MATTER

KW - BRAIN-DEVELOPMENT

KW - VULNERABILITY

KW - ADOLESCENCE

KW - 4-AMINOPYRIDINE

KW - ORGANIZATION

KW - STRUCTURAL-CHANGES

KW - ONTOGENY

KW - NERVOUS-TISSUE

UR - http://www.scopus.com/inward/record.url?scp=85063567951&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/agedependent-electrocorticogram-dynamics-epileptogenic-responsiveness-rats-subjected-prenatal-hypoxi

U2 - 10.1159/000497224

DO - 10.1159/000497224

M3 - Article

VL - 41

SP - 56

EP - 66

JO - Developmental Neuroscience

JF - Developmental Neuroscience

SN - 0378-5866

IS - 1-2

ER -