A quantitative assessment of internal electrostatic fields in bone tissue is conducted within the framework of the “bone-battery” concept. It is shown that their influence on the atomic structure and dynamics is significantly underestimated. To test this hypothesis Raman spectroscopy is applied to cortical bone of young and mature rats. Particular attention is paid to the v1(PO43-)-band at 960 cm-1 in the Raman spectra to study the interference of P - O stretching with the dipole polarization of PO43- ions in the electrostatic fields. The v1(PO43-)-band is found split into the bulk and interface components with predominant Gaussian and Lorentzian shapes respectively. Their formation is a subject of site- and age-dependent dipoles polarization at the surface of nanocrystals. Based on the experimental and theoretical results, both the memory effect during the mineralization of bioapatite nanocrystals and quantum effects associated with low-dimensional quantization of valence states in hydrated nanolayers are predicted, and the prospects for the development of bone tissue engineering at the nanoscale are considered.