A study of extracellular vesicles isolated from blood plasma conducted by low-voltage scanning electron microscopy

K. A. Kondratov, T. A. Petrova, V. Yu Mikhailovskii, A. N. Ivanova, A. A. Kostareva, A. V. Fedorov

    Research output: Contribution to journalArticlepeer-review

    14 Scopus citations

    Abstract

    Extracellular vesicles secreted by cells represent an almost spherical membrane structures enriched with biological molecules of different types. The number and molecular composition of these structures depend on both the physiological state of an organism and underlying diseases. Despite extracellular vesicles playing an important role in intercellular communication and being potential biomarkers of pathological processes, the mechanisms of their formation, their functions, and their morphological characteristics are poorly studied. Low-voltage scanning electron microscopy is a promising method for studying extracellular vesicles, since it does not need a layer of conductive covering and, consequently, permits morphological details of studied objects to be vizualized at a high resolution in a nanometer range. The results of investigation of the morphology and sizes of objects in blood-plasma fractions by low-voltage scanning electron microscopy are presented in this study.

    Original languageEnglish
    Pages (from-to)181-190
    Number of pages10
    JournalCell and Tissue Biology
    Volume11
    Issue number3
    DOIs
    StatePublished - 1 May 2017

    Scopus subject areas

    • Cell Biology

    Keywords

    • blood plasma
    • CD9 protein
    • exosomes
    • extracellular vesicles
    • low-voltage scanning electron microscopy
    • microvesicles
    • transmission electron microscopy

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