A Snapshot of the Plant Glycated Proteome: STRUCTURAL, FUNCTIONAL, AND MECHANISTIC ASPECTS

T. Bilova, E. Lukasheva, D. Brauch, U. Greifenhagen, G. Paudel, E. Tarakhovskaya, N. Frolova, J. Mittasch, G.U. Balcke, A. Tissier, N. Osmolovskaya, T. Vogt, L.A. Wessjohann, C. Birkemeyer, C. Milkowski, A. Frolov

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Glycation is the reaction of carbonyl compounds (reducing sugars and α-dicarbonyls) with amino acids, lipids, and proteins, yielding early and advanced glycation end products (AGEs). The AGEs can be formed via degradation of early glycation intermediates (glycoxidation) and by interaction with the products of monosaccharide autoxidation (autoxidative glycosylation). Although formation of these potentially deleterious compounds is well characterized in animal systems and thermally treated foods, only a little information about advanced glycation in plants is available. Thus, the knowledge of the plant AGE patterns and the underlying pathways of their formation are completely missing. To fill this gap, we describe the AGE-modified proteome ofBrassica napusand characterize individual sites of advanced glycation by the methods of liquid chromatography-based bottom-up proteomics. The modification patterns were complex but reproducible: 789 AGE-modified peptides in 772 proteins were detected in two independent experiments. In contrast, only 168 polypeptides contained early glycated lysines, which did not resemble the sites of advanced glycation. Similar observations were made withArabidopsis thaliana The absence of the early glycated precursors of the AGE-modified protein residues indicated autoxidative glycosylation, but not glycoxidation, as the major pathway of AGE formation. To prove this assumption and to identify the potential modifying agents, we estimated the reactivity and glycative potential of plant-derived sugars using a model peptide approach and liquid chromatography-mass spectrometry-based techniques. Evaluation of these data sets together with the assessed tissue carbohydrate contents revealed dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, ribulose, erythrose, and sucrose as potential precursors of plant AGEs.

Original languageEnglish
Pages (from-to)7621-7636
Number of pages16
JournalJournal of Biological Chemistry
Volume291
Issue number14
DOIs
StatePublished - 2016

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Brassica napus/genetics
  • Glycoproteins/genetics
  • Glycosylation
  • Plant Proteins/genetics
  • Proteome/genetics
  • Proteomics

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