A novel smartphone-based CD-spectrometer for high sensitive and cost-effective colorimetric detection of ascorbic acid

Liubing Kong, Ying Gan, Tao Liang, Longjie Zhong, Yuxiang Pan, Dmitry Kirsanov, Andrey Legin, Hao Wan, Ping Wang

Research output

Abstract

As a powerful tool for medical diagnosis and bioanalysis, conventional optical spectrometers are generally expensive, bulky and always require an accompanying data processing device. In this work, we developed a novel smartphone-based CD-spectrometer (SCDS) for high sensitive and ultra-portable colorimetric analysis, with the advantage of cost-effective and simplicity. The distance between the light source and slit, the structure of SCDS and the parameters of camera in the smartphone were all optimized to ensure the best analytical performance. Besides, the SCDS employed HSV color model and utilized the overall intensity calculated by summing V-value of adjacent position for the absorbance measurement. In this way the errors caused by the low resolution of CD-grating can effectively be eliminated to promote the sensitivity of the SCDS. The performance of the SCDS was first validated for colorimetric detection of BSA with a detection limit of 0.0073 mg/mL, which is superior compared to that of the microtiter plate reader (MTPR). Moreover, by combining with 3,3′,5,5′-tetramethylbenzidine-manganese dioxide (TMB-MnO2) nanosheets reaction, a high sensitive and specific system for ascorbic acid detection was established. The SCDS gives a detection range from 0.6250 μM to 40 μM with a detection limit of 0.4946 μM for AA detection. Compared to other studies, the SCDS features wide detection range and very low detection limit with low cost instrument. Therefore, the SCDS will be an ideal and promising colorimetric system for point-of-care (POC) application in food security, disease diagnosis and environmental monitoring.

Original languageEnglish
Pages (from-to)150-159
Number of pages10
JournalAnalytica Chimica Acta
Volume1093
Early online date2 Oct 2019
DOIs
Publication statusPublished - 6 Jan 2020

Fingerprint

Smartphones
ascorbic acid
Ascorbic Acid
Spectrometers
spectrometer
Costs and Cost Analysis
cost
Costs
Limit of Detection
Colorimetric analysis
Point-of-Care Systems
detection
Smartphone
Food Supply
Nanosheets
Environmental Monitoring
environmental monitoring
food security
absorbance
Light sources

Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Kong, Liubing ; Gan, Ying ; Liang, Tao ; Zhong, Longjie ; Pan, Yuxiang ; Kirsanov, Dmitry ; Legin, Andrey ; Wan, Hao ; Wang, Ping. / A novel smartphone-based CD-spectrometer for high sensitive and cost-effective colorimetric detection of ascorbic acid. In: Analytica Chimica Acta. 2020 ; Vol. 1093. pp. 150-159.
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A novel smartphone-based CD-spectrometer for high sensitive and cost-effective colorimetric detection of ascorbic acid. / Kong, Liubing; Gan, Ying; Liang, Tao; Zhong, Longjie; Pan, Yuxiang; Kirsanov, Dmitry; Legin, Andrey; Wan, Hao; Wang, Ping.

In: Analytica Chimica Acta, Vol. 1093, 06.01.2020, p. 150-159.

Research output

TY - JOUR

T1 - A novel smartphone-based CD-spectrometer for high sensitive and cost-effective colorimetric detection of ascorbic acid

AU - Kong, Liubing

AU - Gan, Ying

AU - Liang, Tao

AU - Zhong, Longjie

AU - Pan, Yuxiang

AU - Kirsanov, Dmitry

AU - Legin, Andrey

AU - Wan, Hao

AU - Wang, Ping

PY - 2020/1/6

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N2 - As a powerful tool for medical diagnosis and bioanalysis, conventional optical spectrometers are generally expensive, bulky and always require an accompanying data processing device. In this work, we developed a novel smartphone-based CD-spectrometer (SCDS) for high sensitive and ultra-portable colorimetric analysis, with the advantage of cost-effective and simplicity. The distance between the light source and slit, the structure of SCDS and the parameters of camera in the smartphone were all optimized to ensure the best analytical performance. Besides, the SCDS employed HSV color model and utilized the overall intensity calculated by summing V-value of adjacent position for the absorbance measurement. In this way the errors caused by the low resolution of CD-grating can effectively be eliminated to promote the sensitivity of the SCDS. The performance of the SCDS was first validated for colorimetric detection of BSA with a detection limit of 0.0073 mg/mL, which is superior compared to that of the microtiter plate reader (MTPR). Moreover, by combining with 3,3′,5,5′-tetramethylbenzidine-manganese dioxide (TMB-MnO2) nanosheets reaction, a high sensitive and specific system for ascorbic acid detection was established. The SCDS gives a detection range from 0.6250 μM to 40 μM with a detection limit of 0.4946 μM for AA detection. Compared to other studies, the SCDS features wide detection range and very low detection limit with low cost instrument. Therefore, the SCDS will be an ideal and promising colorimetric system for point-of-care (POC) application in food security, disease diagnosis and environmental monitoring.

AB - As a powerful tool for medical diagnosis and bioanalysis, conventional optical spectrometers are generally expensive, bulky and always require an accompanying data processing device. In this work, we developed a novel smartphone-based CD-spectrometer (SCDS) for high sensitive and ultra-portable colorimetric analysis, with the advantage of cost-effective and simplicity. The distance between the light source and slit, the structure of SCDS and the parameters of camera in the smartphone were all optimized to ensure the best analytical performance. Besides, the SCDS employed HSV color model and utilized the overall intensity calculated by summing V-value of adjacent position for the absorbance measurement. In this way the errors caused by the low resolution of CD-grating can effectively be eliminated to promote the sensitivity of the SCDS. The performance of the SCDS was first validated for colorimetric detection of BSA with a detection limit of 0.0073 mg/mL, which is superior compared to that of the microtiter plate reader (MTPR). Moreover, by combining with 3,3′,5,5′-tetramethylbenzidine-manganese dioxide (TMB-MnO2) nanosheets reaction, a high sensitive and specific system for ascorbic acid detection was established. The SCDS gives a detection range from 0.6250 μM to 40 μM with a detection limit of 0.4946 μM for AA detection. Compared to other studies, the SCDS features wide detection range and very low detection limit with low cost instrument. Therefore, the SCDS will be an ideal and promising colorimetric system for point-of-care (POC) application in food security, disease diagnosis and environmental monitoring.

KW - Ascorbic acid detection

KW - HSV color model

KW - MnO nanosheets

KW - Point-of-care

KW - Smartphone-based CD-spectrometer

KW - MnO2 nanosheets

KW - PLATFORM

KW - OXIDASE

KW - COMBINATION

KW - CANCER

KW - FOOD

KW - QUANTUM DOTS

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DO - 10.1016/j.aca.2019.09.071

M3 - Article

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SP - 150

EP - 159

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

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