A comparative characterisation of the complete mitochondrial genomes of Paramoeba aparasomata and Neoparamoeba pemaquidensis (Amoebozoa, Paramoebidae)

Natalya Bondarenko, Ekaterina Volkova, Alexey Masharsky, Alexander Kudryavtsev, Alexey Smirnov

Research output

Abstract

Marine amoebae of the genus Paramoeba (Amoebozoa, Dactylopodida) normally contain a eukaryotic endosymbiont known as Perkinsela‐like organism (PLO). This is one of the characters to distinguish the genera Neoparamoeba and Paramoeba from other Dactylopodida. It is known that the PLO may be lost, but PLO‐free strains of paramoebians were never available for molecular studies. Recently, we have described the first species of the genus Paramoeba which has no parasome ‐ Paramoeba aparasomata. In this study we present a mitochondrial genome of this species, compare it with that of Neoparamoeba pemaquidensis and analyze the evolutionary dynamics of gene sequences and gene order rearrangements between these species. The mitochondrial genome of P. aparasomata is 46,254 bp long and contains a set of 31 protein‐coding genes, 19 tRNAs, two rRNA genes and 7 open reading frames. Our results suggest that these two mitochondrial genomes within the genus Paramoeba have rather similar organization and gene order, base composition, codon usage, the composition and structure of non‐coding and overlapping regions.
Original languageEnglish
JournalJournal of Eukaryotic Microbiology
Early online date10 Oct 2019
DOIs
Publication statusE-pub ahead of print - 10 Oct 2019

Fingerprint

Amoebozoa
Mitochondrial Genome
Gene Order
Amoeba
Gene Rearrangement
Base Composition
Transfer RNA
rRNA Genes
Codon
Open Reading Frames
Genes

Cite this

@article{02a0df8a8c8e4e21ae7e62b90f5ecb7e,
title = "A comparative characterisation of the complete mitochondrial genomes of Paramoeba aparasomata and Neoparamoeba pemaquidensis (Amoebozoa, Paramoebidae)",
abstract = "Marine amoebae of the genus Paramoeba (Amoebozoa, Dactylopodida) normally contain a eukaryotic endosymbiont known as Perkinsela‐like organism (PLO). This is one of the characters to distinguish the genera Neoparamoeba and Paramoeba from other Dactylopodida. It is known that the PLO may be lost, but PLO‐free strains of paramoebians were never available for molecular studies. Recently, we have described the first species of the genus Paramoeba which has no parasome ‐ Paramoeba aparasomata. In this study we present a mitochondrial genome of this species, compare it with that of Neoparamoeba pemaquidensis and analyze the evolutionary dynamics of gene sequences and gene order rearrangements between these species. The mitochondrial genome of P. aparasomata is 46,254 bp long and contains a set of 31 protein‐coding genes, 19 tRNAs, two rRNA genes and 7 open reading frames. Our results suggest that these two mitochondrial genomes within the genus Paramoeba have rather similar organization and gene order, base composition, codon usage, the composition and structure of non‐coding and overlapping regions.",
keywords = "Paramoeba, Amoebozoa, Dactylopodida, Mitochondrial genome, Mitochondrion",
author = "Natalya Bondarenko and Ekaterina Volkova and Alexey Masharsky and Alexander Kudryavtsev and Alexey Smirnov",
year = "2019",
month = "10",
day = "10",
doi = "10.1111/jeu.12767",
language = "English",
journal = "Journal of Eukaryotic Microbiology",
issn = "1066-5234",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - A comparative characterisation of the complete mitochondrial genomes of Paramoeba aparasomata and Neoparamoeba pemaquidensis (Amoebozoa, Paramoebidae)

AU - Bondarenko, Natalya

AU - Volkova, Ekaterina

AU - Masharsky, Alexey

AU - Kudryavtsev, Alexander

AU - Smirnov, Alexey

PY - 2019/10/10

Y1 - 2019/10/10

N2 - Marine amoebae of the genus Paramoeba (Amoebozoa, Dactylopodida) normally contain a eukaryotic endosymbiont known as Perkinsela‐like organism (PLO). This is one of the characters to distinguish the genera Neoparamoeba and Paramoeba from other Dactylopodida. It is known that the PLO may be lost, but PLO‐free strains of paramoebians were never available for molecular studies. Recently, we have described the first species of the genus Paramoeba which has no parasome ‐ Paramoeba aparasomata. In this study we present a mitochondrial genome of this species, compare it with that of Neoparamoeba pemaquidensis and analyze the evolutionary dynamics of gene sequences and gene order rearrangements between these species. The mitochondrial genome of P. aparasomata is 46,254 bp long and contains a set of 31 protein‐coding genes, 19 tRNAs, two rRNA genes and 7 open reading frames. Our results suggest that these two mitochondrial genomes within the genus Paramoeba have rather similar organization and gene order, base composition, codon usage, the composition and structure of non‐coding and overlapping regions.

AB - Marine amoebae of the genus Paramoeba (Amoebozoa, Dactylopodida) normally contain a eukaryotic endosymbiont known as Perkinsela‐like organism (PLO). This is one of the characters to distinguish the genera Neoparamoeba and Paramoeba from other Dactylopodida. It is known that the PLO may be lost, but PLO‐free strains of paramoebians were never available for molecular studies. Recently, we have described the first species of the genus Paramoeba which has no parasome ‐ Paramoeba aparasomata. In this study we present a mitochondrial genome of this species, compare it with that of Neoparamoeba pemaquidensis and analyze the evolutionary dynamics of gene sequences and gene order rearrangements between these species. The mitochondrial genome of P. aparasomata is 46,254 bp long and contains a set of 31 protein‐coding genes, 19 tRNAs, two rRNA genes and 7 open reading frames. Our results suggest that these two mitochondrial genomes within the genus Paramoeba have rather similar organization and gene order, base composition, codon usage, the composition and structure of non‐coding and overlapping regions.

KW - Paramoeba

KW - Amoebozoa

KW - Dactylopodida

KW - Mitochondrial genome

KW - Mitochondrion

UR - https://www.ncbi.nlm.nih.gov/pubmed/31600008

U2 - 10.1111/jeu.12767

DO - 10.1111/jeu.12767

M3 - Article

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

ER -