The article deals with the French colonization of the Atlantic region of Canada (the Acadia colony). It focuses on the events of the late 1600s and early 1610s, when the colony was re-established by Jean de Poutrincourt. During this period, a significant interest in Acadia was shown by the Jesuits, who considered it as a stronghold for missionary activity among the Amerindians. It is shown that although Poutrincourt was a proponent of the appearance of the Jesuits in the colonyand sought to avoid it, in the end he was forced to accept it, since they had material and administrative resources that he did not have.