Экспрессия кисспептина и матриксных металлопротеиназ в культуре эндометрия человека: исследование инвазивных и миграционных свойств.

Татьяна Сергеевна Клейменова, Анна Олеговна Дробинцева, Виктория Олеговна Полякова, Анна Алексеевна Цыпурдеева

Research output: Contribution to journalArticle

Abstract

Hypothesis/aims of study. Kisspeptin (KISS1) is encoded by KISS1 gene and its interaction with KISS1 receptor (KISS1R) suppresses metastasis and regulates release of gonadotropin-releasing hormone, which promotes secretion of estradiol and progesterone. Steroid hormone synthesis is regulated by KISS1/KISS1R and its activation can be involved in hormone dependent disorders such as endometriosis. KISS1 expression has been shown to inhibit the activity of a number of matrix metalloproteinases (MMPs). In this study, we aimed to isolate endometrial cell cultures from patients with and without endometriosis; to evaluate KISS1, KISS1R, MMP-2, and MMP-9 protein expression by immunocytochemistry; and to perform culture tests: the scratch assay and the analysis of cell migration activity. Results. It was found that the endometrial cell cultures expressed KISS1, KISS1R, MMP-2, and MMP-9 proteins, with the cell migration ability enhanced.
Original languageRussian
Pages (from-to)43-50
JournalЖУРНАЛ АКУШЕРСТВА И ЖЕНСКИХ БОЛЕЗНЕЙ
Volume68
Issue number2
DOIs
StatePublished - 2019

Keywords

  • cell migration
  • external genital endometriosis
  • human endometrial culture
  • Kiss1r
  • kisspeptin
  • matrix metalloproteinases
  • кисспептин
  • матриксные металлопротеиназы
  • миграция клеток
  • наружный генитальный эндометриоз
  • рецептор кисспептина
  • эндометриальная культура клеток

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