Цистатионин β-синтаза в мозге форели Oncorhynchus mykiss при одностороннем повреждении глаза и в условиях культивирования in vitro.

Е.В. Пущина, А.А. Вараксин, Д.К. Обухов

Research output: Contribution to journalArticle

Abstract

Expression of cystathionine β-synthase (CBS) in the brain of adult trout under normal conditions and at 1 week after eye injury was assessed using Western blot analysis. The study of CBS distribution in the brain of intact trout and after a mechanical eye injury in the telencephalon, tectum, cerebellum, and brainstem was carried out by the method of immunoperoxidase labeling on free-floating sections. The results of the study showed an increase in CBS expression in different brain divisions after eye injury. In the visual projection center of the brain (tectum), radial glia cells expressing CBS were revealed after the injury. The emergence of CBS+ heterogeneous radial glia in the trout tectum after eye injury indicates the ability of the reactive neural stem cells (NSC) to synthesize hydrogen sulfide. CBS+ fibers and cells were found in the proliferative zones of cerebellum (valvula) and telencephalon (ventral region). In the intact trout tegmentum, CBS expression was observed in large neurons of the dorsal r
Original languageRussian
Pages (from-to)84-105
JournalОнтогенез
Volume50
Issue number2
StatePublished - 2019
Externally publishedYes

Keywords

  • cystathionine β -synthase
  • hydrogen sulfide
  • neurosphere-like complex
  • optic nerve
  • primary cell culture
  • radial glia
  • reparative neurogenesis
  • зрительный нерв
  • нейросфероподобный комплекс
  • первичная культура клеток
  • радиальная глия
  • репаративный нейрогенез
  • сероводород
  • цистатионин β-синтаза

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