Способ количественного определения хлорида 2-[(Z)-1-(3,5-дифенил-1,3,4-тиадиазол-2(3Н)-илиден)метил]-3,5-дифенил-1,3,4-тиадиазол-3-ия в биологических объектах.

Надежда Юрьевна Сипкина (Inventor), Игорь Павлович Яковлев (Inventor)

Research output: Patenting and IP registrationPatent

Abstract

<p num="40">FIELD: pharmaceuticals.</p> <p num="41">SUBSTANCE: invention relates to the analytical and pharmaceutical industry, specifically to the determination of residual amounts of chloride 2-[(Z)-1-(3,5-diphenyl-1,3,4-thiadiazole-2(3H)-ylidene)methyl]-3,5-diphenyl-1,3,4-thiadiazole-3-ium (TDZ) in biological fluids in establishing pharmacokinetic parameters and determining a therapeutic dose of the preparation. Disclosed is a method of determining TDZ in biological fluids using high-performance liquid chromatography, involving sampling, precipitation, centrifugation, filtration, introducing the sample into a liquid chromatograph, chromatography and analysis results. Fluorometric detector is used, chromatograms are recorded at excitation wavelength of 435 nm and emission wavelength of 520 nm, Zorbax Eclipse Plus C8 column is used with length of 250 mm, inner diameter of 4.6 mm and particle size of 5 mcm at mobile phase flow rate of 1.0 ml/min and volume of introduced sample 20 mcl.</p> <p num="42">EFFE
Original languageRussian
Patent numberRU 2702330
StatePublished - 25 Jun 2019
Externally publishedYes

Cite this