Prospects for the Application of Yeast Display in Biotechnology and Cell Biology (Review)

Research output

2 Citations (Scopus)

Abstract

The technology of the yeast cell surface display, which appeared 20 years ago and was based on the displaying of target proteins on the cell surface via fusion to an abundant cell wall protein finds broad application in basic and applied research. The main advantage of the cell surface display on the basis of eukaryotic microorganisms—yeast—is the opportunity for correct modification of mammalian proteins. The cell surface display is an important tool for the analysis and understanding of protein function and protein–protein interactions and for the screening of novel clones from peptide and protein libraries. This technology makes it possible to obtain cells with novel abilities, such as catalytic functions and affinity binding to valuable ligands, including rare and heavy metals. It provides the chance to use yeast in biotechnology and in bioremediation and biomonitoring of the environment. The review considers the methods of obtaining a cell surface display on the basis of the yeasts Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica, the properties of anchor proteins, and the main fields of yeast display technology.

Original languageEnglish
Pages (from-to)337-351
Number of pages15
JournalApplied Biochemistry and Microbiology
Volume54
Issue number4
DOIs
Publication statusPublished - 1 Jul 2018

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Cytology
Biotechnology
Yeast
Cell Biology
Yeasts
Display devices
Proteins
Cell Surface Display Techniques
Yarrowia
Technology
Peptide Library
Cells
Environmental Biodegradation
Pichia
Environmental Monitoring
Heavy Metals
Bioremediation
Cell Wall
Saccharomyces cerevisiae
Anchors

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

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title = "Prospects for the Application of Yeast Display in Biotechnology and Cell Biology (Review)",
abstract = "The technology of the yeast cell surface display, which appeared 20 years ago and was based on the displaying of target proteins on the cell surface via fusion to an abundant cell wall protein finds broad application in basic and applied research. The main advantage of the cell surface display on the basis of eukaryotic microorganisms—yeast—is the opportunity for correct modification of mammalian proteins. The cell surface display is an important tool for the analysis and understanding of protein function and protein–protein interactions and for the screening of novel clones from peptide and protein libraries. This technology makes it possible to obtain cells with novel abilities, such as catalytic functions and affinity binding to valuable ligands, including rare and heavy metals. It provides the chance to use yeast in biotechnology and in bioremediation and biomonitoring of the environment. The review considers the methods of obtaining a cell surface display on the basis of the yeasts Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica, the properties of anchor proteins, and the main fields of yeast display technology.",
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