Изучение маркера активного хроматина - ацетилированного гистона н3к9 - хромосом из лимфоцитов человека в пре- и постнатальный период онтогенеза.

Ольга Алексеевна Ефимова, Анна Андреевна Пендина, Юлия Георгиевна Лежнина, Андрей Владимирович Тихонов, Ольга Гавриловна Чиряева, Любовь Ивановна Петрова, Вера Святославовна Дудкина, Алла Сергеевна Кольцова, Михаил Игоревич Крапивин, Анастасия Васильевна Петровская-Каминская, Ольга Евгеньевна Талантова, Татьяна Владимировна Кузнецова, Владислав Сергеевич Баранов

Research output: Contribution to journalArticle

Abstract

Background: Incorrect epigenetic modifications of the human genome may result in epigenetic disorders, thus, highlighting the necessity of studying chromosome epigenetic patterns in human development. Aim of the study: A comparative analysis of acetylated histone H3K9 (AcH3K9) patterns in human metaphase chromosomes from the lymphocytes of adults and fetuses. Materials and methods: The immunocytochemical detection of AcH3K9 in the metaphase chromosomes from PHA-stimulated peripheral lymphocytes of 13 adults and cord blood lymphocytes of 10 fetuses at 20-22 weeks of gestation. Results: Both in the chromosomes of the adults and the fetuses, AcH3K9 accumulated in the R- and T-, but not G-bands and avoided the regions of pericentromeric heterochromatin of the chromosomes 1, 9 and 16. When comparing the adult and the fetal chromosomes, different levels of AcH3K9 were revealed in a few bands: 2q31, 5p13, 5p15 and 16p13 had higher level of Н3К9 acetylation in adults, in contrast to 9q13 which was hyperacetylated in
Original languageRussian
Pages (from-to)111-117
JournalЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА
Volume17
Issue number3
StatePublished - 2019
Externally publishedYes

Keywords

  • chromatin disorders
  • chromosomes
  • epigenetics
  • histone acetylation
  • human development
  • prenatal diagnosis
  • ацетилирование гистонов
  • онтогенез человека
  • пренатальная диагностика
  • хроматиновые болезни
  • хромосомы
  • эпигенетика

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