Are there any species-specific sperm proteins in periwinkles?

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Population ranges of different Littorina species overlap, allowing not species-specific copulations and incomplete reproductive isolation between some of them. This poses question how these coexisting related species maintain their identity. Gamete incompatibility is one of the possible mechanisms of
reproductive isolation in such system. In this case, a search for acrosomal
proteins that are responsible for the specificity of fertilization can be a promising direction. In some taxa (e.g. abalones and sea urchins) species-specific acrosomal proteins were studied. Mutations in these proteins are supposed to cause speciation in these groups. Thus, the search for acrosomal proteins advances our understanding of the reproductive isolation mechanisms and speciation in genus Littorina. For present study we obtained reproductive tissue extracts, total lysates of different body parts and phenolic fixations for 5 periwinkle species (L.obtusata, L.littorea, L.saxatilis, L.arcana, L.compressa), and acrosomal extract only for L.obtusata. The protein samples were analyzed using proteomics methods (1D and 2D-electrophoresis and «bottom up» analysis). We were able to identify a sperm-specific protein of L.obtusata with an approximate
weight of 17 kDa. Edman sequencing allowed identifying its first 10 amino acids. Based on this sequence we made degenerate primers and used them for 3'-RACE. Finally, we identified a reproductive-specific transcript of the target protein that shows no homology with any known proteins. This protein is a species-specific marker of L.obtusata. Its acrosomal localization and
involvement into reproductive isolation process need further examination
Translated title of the contributionЕсть ли какие-либо видоспецифичные спермальные белки у литоральных моллюсков?
Original languageEnglish
Number of pages48
StatePublished - 28 Jul 2014
EventInternationol Symposium on Littorinid Biology and Evolution - Hong Kong, China
Duration: 28 Jul 20141 Aug 2014
Conference number: 11


ConferenceInternationol Symposium on Littorinid Biology and Evolution
CityHong Kong
Internet address

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