Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold

T. Yu. Lvova, O. I. Stepanova, L. P. Viazmina, L. S. Okorokova, Ksenia Lvovna Belyakova, M. E. Belikova, Sergey Alekseevich Selkov, D. I. Sokolov

Research output

1 Citation (Scopus)

Abstract

We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalBulletin of Experimental Biology and Medicine
Volume161
Issue number1
DOIs
Publication statusPublished - 2016
Externally publishedYes

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Scaffolds
Placenta
Cultured Cells
Phenotype
Molecules
Third Pregnancy Trimester
First Pregnancy Trimester
Pre-Eclampsia
Pregnancy
Cells
matrigel

Cite this

Lvova, T. Yu. ; Stepanova, O. I. ; Viazmina, L. P. ; Okorokova, L. S. ; Belyakova, Ksenia Lvovna ; Belikova, M. E. ; Selkov, Sergey Alekseevich ; Sokolov, D. I. / Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold. In: Bulletin of Experimental Biology and Medicine. 2016 ; Vol. 161, No. 1. pp. 162-167.
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title = "Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold",
abstract = "We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.",
keywords = "surface receptors, macrophages, preeclampsia, placenta, IN-VITRO, ADHESION MOLECULES, TNF-ALPHA, MACROPHAGES, ANGIOGENESIS, PREGNANCY, MONOCYTES, DIFFERENTIATION, VASCULOGENESIS, PREECLAMPSIA",
author = "Lvova, {T. Yu.} and Stepanova, {O. I.} and Viazmina, {L. P.} and Okorokova, {L. S.} and Belyakova, {Ksenia Lvovna} and Belikova, {M. E.} and Selkov, {Sergey Alekseevich} and Sokolov, {D. I.}",
year = "2016",
doi = "10.1007/s10517-016-3368-4",
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Lvova, TY, Stepanova, OI, Viazmina, LP, Okorokova, LS, Belyakova, KL, Belikova, ME, Selkov, SA & Sokolov, DI 2016, 'Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold', Bulletin of Experimental Biology and Medicine, vol. 161, no. 1, pp. 162-167. https://doi.org/10.1007/s10517-016-3368-4

Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold. / Lvova, T. Yu.; Stepanova, O. I.; Viazmina, L. P.; Okorokova, L. S.; Belyakova, Ksenia Lvovna; Belikova, M. E.; Selkov, Sergey Alekseevich; Sokolov, D. I.

In: Bulletin of Experimental Biology and Medicine, Vol. 161, No. 1, 2016, p. 162-167.

Research output

TY - JOUR

T1 - Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold

AU - Lvova, T. Yu.

AU - Stepanova, O. I.

AU - Viazmina, L. P.

AU - Okorokova, L. S.

AU - Belyakova, Ksenia Lvovna

AU - Belikova, M. E.

AU - Selkov, Sergey Alekseevich

AU - Sokolov, D. I.

PY - 2016

Y1 - 2016

N2 - We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.

AB - We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.

KW - surface receptors

KW - macrophages

KW - preeclampsia

KW - placenta

KW - IN-VITRO

KW - ADHESION MOLECULES

KW - TNF-ALPHA

KW - MACROPHAGES

KW - ANGIOGENESIS

KW - PREGNANCY

KW - MONOCYTES

KW - DIFFERENTIATION

KW - VASCULOGENESIS

KW - PREECLAMPSIA

U2 - 10.1007/s10517-016-3368-4

DO - 10.1007/s10517-016-3368-4

M3 - статья

C2 - 27259498

VL - 161

SP - 162

EP - 167

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

IS - 1

ER -