Abstract

An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid
Original languageEnglish
Article numberDOI: 10.1016/j.talanta.2018.02.112
Pages (from-to)122-127
Number of pages6
JournalTalanta
Volume184
Issue number1 July
Publication statusPublished - 2018

Scopus subject areas

  • Environmental Science(all)

Cite this

@article{da738853246f4c3c8ad16dc01feb7cba,
title = "An automated salting-out assisted liquid-liquid microextraction approach using 1-octylamine: On-line separation of tetracycline in urine samples followed by HPLC-UV determination",
abstract = "An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid",
keywords = "1-Octylamine, Flow analysis, HPLC-UV, Salting-out assisted liquid-liquid microextraction, Tetracycline, urine",
author = "Кристина Вах and Булатов, {Андрей Васильевич} and Черкашина, {Ксения Дмитриевна} and Москвин, {Леонид Николаевич} and Лезов, {Алексей Андреевич} and Лебединец, {Софья Андреевна} and Почивалов, {Алексей Сергеевич}",
note = "6 страниц, 30 ссылок",
year = "2018",
language = "English",
volume = "184",
pages = "122--127",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "1 July",

}

TY - JOUR

T1 - An automated salting-out assisted liquid-liquid microextraction approach using 1-octylamine: On-line separation of tetracycline in urine samples followed by HPLC-UV determination

AU - Вах, Кристина

AU - Булатов, Андрей Васильевич

AU - Черкашина, Ксения Дмитриевна

AU - Москвин, Леонид Николаевич

AU - Лезов, Алексей Андреевич

AU - Лебединец, Софья Андреевна

AU - Почивалов, Алексей Сергеевич

N1 - 6 страниц, 30 ссылок

PY - 2018

Y1 - 2018

N2 - An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid

AB - An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid

KW - 1-Octylamine, Flow analysis, HPLC-UV, Salting-out assisted liquid-liquid microextraction, Tetracycline, urine

M3 - Article

VL - 184

SP - 122

EP - 127

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 1 July

M1 - DOI: 10.1016/j.talanta.2018.02.112

ER -