Abstract
| Original language | English |
|---|---|
| Article number | DOI: 10.1016/j.talanta.2018.02.112 |
| Pages (from-to) | 122-127 |
| Number of pages | 6 |
| Journal | Talanta |
| Volume | 184 |
| Issue number | 1 July |
| Publication status | Published - 2018 |
Scopus subject areas
- Environmental Science(all)
Cite this
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An automated salting-out assisted liquid-liquid microextraction approach using 1-octylamine: On-line separation of tetracycline in urine samples followed by HPLC-UV determination. / Вах, Кристина; Булатов, Андрей Васильевич; Черкашина, Ксения Дмитриевна; Москвин, Леонид Николаевич; Лезов, Алексей Андреевич; Лебединец, Софья Андреевна; Почивалов, Алексей Сергеевич.
In: Talanta, Vol. 184, No. 1 July, DOI: 10.1016/j.talanta.2018.02.112, 2018, p. 122-127.Research output
TY - JOUR
T1 - An automated salting-out assisted liquid-liquid microextraction approach using 1-octylamine: On-line separation of tetracycline in urine samples followed by HPLC-UV determination
AU - Вах, Кристина
AU - Булатов, Андрей Васильевич
AU - Черкашина, Ксения Дмитриевна
AU - Москвин, Леонид Николаевич
AU - Лезов, Алексей Андреевич
AU - Лебединец, Софья Андреевна
AU - Почивалов, Алексей Сергеевич
N1 - 6 страниц, 30 ссылок
PY - 2018
Y1 - 2018
N2 - An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid
AB - An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid
KW - 1-Octylamine, Flow analysis, HPLC-UV, Salting-out assisted liquid-liquid microextraction, Tetracycline, urine
M3 - Article
VL - 184
SP - 122
EP - 127
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1 July
M1 - DOI: 10.1016/j.talanta.2018.02.112
ER -